points tools-events Search Results


v10 software  (Becton Dickinson)
90
Becton Dickinson v10 software
Autophagy induced by milk-δVB. ( a ) Representative images of green detection reagent imaged by confocal laser microscopy after 72 h in LoVo cells incubated with 40% v/v buffalo milk (milk), δVB (2 mM) and milk supplemented with δVB (milk + δVB). Scale bar, 50 μm. ( b ) As for flow cytometry analysis, at least 10.000 events were acquired in log mode. For the quantitative evaluation of green detection reagent, FlowJo <t>V10</t> software was used to calculate median fluorescence intensities. ( c ) Bar graph of the fluorescence intensity values of green detection reagent-labeled vesicles and expressed as fold change of the control (Ctr). Analysis was carried out by determinations in triplicate of n = 3 experiments ** P < 0.01 vs Ctr, † P < 0.05 vs milk, # P < 0.05 vs δVB. ( d – g ) Protein expression levels of LC3BII/ LC3BI, p62, Atg7 and Beclin 1, measured by Western blot in control LoVo cells (Ctr) or cells treated for 72 h with milk (40% v/v), δVB (2 mM) and milk+δVB. Lane 1 = Ctr, lane 2 = milk, lane 3 = δVB, lane 4 = milk + δVB. Protein content was calculated with Image J software 1.52n version and expressed as arbitrary units (AU). * P < 0.05 vs Ctr; ** P < 0.01 vs Ctr; †† P < 0.01 vs milk. The full-length blots are showed in the supplementary information (Fig. ).
V10 Software, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/points+tools-events/v10+software/pmc07265370-219-18-21
Average 90 stars, based on 1 article reviews
v10 software - by Bioz Stars, 2026-09
90/100 stars
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cellquest software  (Becton Dickinson)
90
Becton Dickinson cellquest software
Autophagy induced by milk-δVB. ( a ) Representative images of green detection reagent imaged by confocal laser microscopy after 72 h in LoVo cells incubated with 40% v/v buffalo milk (milk), δVB (2 mM) and milk supplemented with δVB (milk + δVB). Scale bar, 50 μm. ( b ) As for flow cytometry analysis, at least 10.000 events were acquired in log mode. For the quantitative evaluation of green detection reagent, FlowJo <t>V10</t> software was used to calculate median fluorescence intensities. ( c ) Bar graph of the fluorescence intensity values of green detection reagent-labeled vesicles and expressed as fold change of the control (Ctr). Analysis was carried out by determinations in triplicate of n = 3 experiments ** P < 0.01 vs Ctr, † P < 0.05 vs milk, # P < 0.05 vs δVB. ( d – g ) Protein expression levels of LC3BII/ LC3BI, p62, Atg7 and Beclin 1, measured by Western blot in control LoVo cells (Ctr) or cells treated for 72 h with milk (40% v/v), δVB (2 mM) and milk+δVB. Lane 1 = Ctr, lane 2 = milk, lane 3 = δVB, lane 4 = milk + δVB. Protein content was calculated with Image J software 1.52n version and expressed as arbitrary units (AU). * P < 0.05 vs Ctr; ** P < 0.01 vs Ctr; †† P < 0.01 vs milk. The full-length blots are showed in the supplementary information (Fig. ).
Cellquest Software, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/points+tools-events/cellquest+software/pm23161408-74-39-41
Average 90 stars, based on 1 article reviews
cellquest software - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
cellqueste software  (Becton Dickinson)
90
Becton Dickinson cellqueste software
Autophagy induced by milk-δVB. ( a ) Representative images of green detection reagent imaged by confocal laser microscopy after 72 h in LoVo cells incubated with 40% v/v buffalo milk (milk), δVB (2 mM) and milk supplemented with δVB (milk + δVB). Scale bar, 50 μm. ( b ) As for flow cytometry analysis, at least 10.000 events were acquired in log mode. For the quantitative evaluation of green detection reagent, FlowJo <t>V10</t> software was used to calculate median fluorescence intensities. ( c ) Bar graph of the fluorescence intensity values of green detection reagent-labeled vesicles and expressed as fold change of the control (Ctr). Analysis was carried out by determinations in triplicate of n = 3 experiments ** P < 0.01 vs Ctr, † P < 0.05 vs milk, # P < 0.05 vs δVB. ( d – g ) Protein expression levels of LC3BII/ LC3BI, p62, Atg7 and Beclin 1, measured by Western blot in control LoVo cells (Ctr) or cells treated for 72 h with milk (40% v/v), δVB (2 mM) and milk+δVB. Lane 1 = Ctr, lane 2 = milk, lane 3 = δVB, lane 4 = milk + δVB. Protein content was calculated with Image J software 1.52n version and expressed as arbitrary units (AU). * P < 0.05 vs Ctr; ** P < 0.01 vs Ctr; †† P < 0.01 vs milk. The full-length blots are showed in the supplementary information (Fig. ).
Cellqueste Software, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/points+tools-events/cellqueste+software/10__1667_slash_rr14014__1-83-27-29
Average 90 stars, based on 1 article reviews
cellqueste software - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Autophagy induced by milk-δVB. ( a ) Representative images of green detection reagent imaged by confocal laser microscopy after 72 h in LoVo cells incubated with 40% v/v buffalo milk (milk), δVB (2 mM) and milk supplemented with δVB (milk + δVB). Scale bar, 50 μm. ( b ) As for flow cytometry analysis, at least 10.000 events were acquired in log mode. For the quantitative evaluation of green detection reagent, FlowJo V10 software was used to calculate median fluorescence intensities. ( c ) Bar graph of the fluorescence intensity values of green detection reagent-labeled vesicles and expressed as fold change of the control (Ctr). Analysis was carried out by determinations in triplicate of n = 3 experiments ** P < 0.01 vs Ctr, † P < 0.05 vs milk, # P < 0.05 vs δVB. ( d – g ) Protein expression levels of LC3BII/ LC3BI, p62, Atg7 and Beclin 1, measured by Western blot in control LoVo cells (Ctr) or cells treated for 72 h with milk (40% v/v), δVB (2 mM) and milk+δVB. Lane 1 = Ctr, lane 2 = milk, lane 3 = δVB, lane 4 = milk + δVB. Protein content was calculated with Image J software 1.52n version and expressed as arbitrary units (AU). * P < 0.05 vs Ctr; ** P < 0.01 vs Ctr; †† P < 0.01 vs milk. The full-length blots are showed in the supplementary information (Fig. ).

Journal: Scientific Reports

Article Title: ROS-Mediated Apoptotic Cell Death of Human Colon Cancer LoVo Cells by Milk δ-Valerobetaine

doi: 10.1038/s41598-020-65865-6

Figure Lengend Snippet: Autophagy induced by milk-δVB. ( a ) Representative images of green detection reagent imaged by confocal laser microscopy after 72 h in LoVo cells incubated with 40% v/v buffalo milk (milk), δVB (2 mM) and milk supplemented with δVB (milk + δVB). Scale bar, 50 μm. ( b ) As for flow cytometry analysis, at least 10.000 events were acquired in log mode. For the quantitative evaluation of green detection reagent, FlowJo V10 software was used to calculate median fluorescence intensities. ( c ) Bar graph of the fluorescence intensity values of green detection reagent-labeled vesicles and expressed as fold change of the control (Ctr). Analysis was carried out by determinations in triplicate of n = 3 experiments ** P < 0.01 vs Ctr, † P < 0.05 vs milk, # P < 0.05 vs δVB. ( d – g ) Protein expression levels of LC3BII/ LC3BI, p62, Atg7 and Beclin 1, measured by Western blot in control LoVo cells (Ctr) or cells treated for 72 h with milk (40% v/v), δVB (2 mM) and milk+δVB. Lane 1 = Ctr, lane 2 = milk, lane 3 = δVB, lane 4 = milk + δVB. Protein content was calculated with Image J software 1.52n version and expressed as arbitrary units (AU). * P < 0.05 vs Ctr; ** P < 0.01 vs Ctr; †† P < 0.01 vs milk. The full-length blots are showed in the supplementary information (Fig. ).

Article Snippet: For the evaluation of intracellular DNA content, at least 10.000 events for each point were analyzed with the FlowJo V10 software (FlowJo LLC, USA) ( www.flowjo.com/solutions/flowjo ).

Techniques: Microscopy, Incubation, Flow Cytometry, Software, Fluorescence, Labeling, Expressing, Western Blot

Suppression of ROS production. ( a,b ) Flow cytometry analysis of green detection reagent staining following incubation with δVB (2 mM) and δVB + NAC. ** P < 0.01 vs Ctr, °P < 0.01 v s δVB. ( c, d ) Arbitrary units (AU) of SIRT6 protein expression in cells treated with δVB and milk+δVB in the presence of NAC. Lane 1 = Ctr, lane 2 = NAC + δVB, lane 3 = NAC + milk + δVB, * P < 0.05 vs Ctr. ( e ) SIRT6 protein expression in c e lls treated with RNAifectin transfection reagent (Vehicle), scramble siRNA (50 nM) (Scramble), SIRT6-siRNA (50 nM) or medium only (Ctr). Lane 1 = Ctr, lane 2 = Vehicle, lane 3 = Scramble, lane 4 = SIRT6-siRNA. ( f,g ) Flow cytometric autophagic activity performed by green detection reagent in LoVo cells treated for 72 h with milk+δVB, SIRT6-siRNA+milk+ δVB, or HBSS-10 mM Hepes (40% v/v) (Ctr). Rapamycin (1 μM) was used as positive control. FlowJo V10 software was used to calculate median fluorescence intensities. ( h) Protein expression levels of LC3BI/ LC3BII from LoVo cells treated for 72 h with milk + δVB, SIRT6-siRNA + milk + δVB, or HBSS-10 mM Hepes (40% v/v) (Ctr). Lane 1 = Ctr, lane 2 = milk + δVB, lane 3 = SIRT6-siRNA + milk + δVB. ( i,j ) Percentage of apoptosis and representative dot plots of annexin V-FITC and PI-stained cells analyzed by flow cytometry in LoVo cells after 72 h of treatment with milk+δVB, SIRT6-siRNA+milk+δVB, or HBSS-10 mM Hepes (40% v/v) (Ctr). Data are expressed as mean ± SD of n = 4 experiments. At least 10.000 events were acquired. ( k ) Protein expression levels of PARP from LoVo cells treated for 72 h with milk + δVB, SIRT6-siRNA + milk + δVB, or HBSS-10 mM Hepes (40% v/v) (Ctr). Lane 1 = Ctr, lane 2 = milk + δVB, Lane 3 = SIRT6 siRNA + milk + δVB. * P < 0.05 vs Ctr, ** P < 0.01 vs Ctr, + P < 0.05 vs milk + δVB, ++ P < 0.01 vs milk + δVB.

Journal: Scientific Reports

Article Title: ROS-Mediated Apoptotic Cell Death of Human Colon Cancer LoVo Cells by Milk δ-Valerobetaine

doi: 10.1038/s41598-020-65865-6

Figure Lengend Snippet: Suppression of ROS production. ( a,b ) Flow cytometry analysis of green detection reagent staining following incubation with δVB (2 mM) and δVB + NAC. ** P < 0.01 vs Ctr, °P < 0.01 v s δVB. ( c, d ) Arbitrary units (AU) of SIRT6 protein expression in cells treated with δVB and milk+δVB in the presence of NAC. Lane 1 = Ctr, lane 2 = NAC + δVB, lane 3 = NAC + milk + δVB, * P < 0.05 vs Ctr. ( e ) SIRT6 protein expression in c e lls treated with RNAifectin transfection reagent (Vehicle), scramble siRNA (50 nM) (Scramble), SIRT6-siRNA (50 nM) or medium only (Ctr). Lane 1 = Ctr, lane 2 = Vehicle, lane 3 = Scramble, lane 4 = SIRT6-siRNA. ( f,g ) Flow cytometric autophagic activity performed by green detection reagent in LoVo cells treated for 72 h with milk+δVB, SIRT6-siRNA+milk+ δVB, or HBSS-10 mM Hepes (40% v/v) (Ctr). Rapamycin (1 μM) was used as positive control. FlowJo V10 software was used to calculate median fluorescence intensities. ( h) Protein expression levels of LC3BI/ LC3BII from LoVo cells treated for 72 h with milk + δVB, SIRT6-siRNA + milk + δVB, or HBSS-10 mM Hepes (40% v/v) (Ctr). Lane 1 = Ctr, lane 2 = milk + δVB, lane 3 = SIRT6-siRNA + milk + δVB. ( i,j ) Percentage of apoptosis and representative dot plots of annexin V-FITC and PI-stained cells analyzed by flow cytometry in LoVo cells after 72 h of treatment with milk+δVB, SIRT6-siRNA+milk+δVB, or HBSS-10 mM Hepes (40% v/v) (Ctr). Data are expressed as mean ± SD of n = 4 experiments. At least 10.000 events were acquired. ( k ) Protein expression levels of PARP from LoVo cells treated for 72 h with milk + δVB, SIRT6-siRNA + milk + δVB, or HBSS-10 mM Hepes (40% v/v) (Ctr). Lane 1 = Ctr, lane 2 = milk + δVB, Lane 3 = SIRT6 siRNA + milk + δVB. * P < 0.05 vs Ctr, ** P < 0.01 vs Ctr, + P < 0.05 vs milk + δVB, ++ P < 0.01 vs milk + δVB.

Article Snippet: For the evaluation of intracellular DNA content, at least 10.000 events for each point were analyzed with the FlowJo V10 software (FlowJo LLC, USA) ( www.flowjo.com/solutions/flowjo ).

Techniques: Flow Cytometry, Staining, Incubation, Expressing, Transfection, Activity Assay, Positive Control, Software, Fluorescence